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Addgene

pFUChW Parkin shRNA Mul1 shRNA
(Plasmid #78523)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 78523 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pFUChW
  • Backbone size w/o insert (bp) 3963
  • Total vector size (bp) 4539
  • Modifications to backbone
    Additional H1 added
  • Vector type
    Lentiviral, RNAi

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Park2 shRNA, Mul1 shRNA
  • gRNA/shRNA sequence
    CCTCCAAGGAAACCATCAA, GAGCTGTGCGGTCTGTTAA
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Prkn (a.k.a. Park2)
  • Entrez Gene
    Mul1 (a.k.a. 0610009K11Rik, AV000801, Gide)
  • Promoter H1

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI, XbaI (unknown if destroyed)
  • 3′ cloning site EcoRI, NheI (unknown if destroyed)
  • 5′ sequencing primer GTCGCTATGTGTTCTGGGAAATCACC
  • 3′ sequencing primer GTCGCTATGTGTTCTGGGAAATCACC
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    modified from pFUGW-h1 (Addgene plasmid 25870)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFUChW Parkin shRNA Mul1 shRNA was a gift from David Chan (Addgene plasmid # 78523 ; http://n2t.net/addgene:78523 ; RRID:Addgene_78523)
  • For your References section:

    Elimination of paternal mitochondria in mouse embryos occurs through autophagic degradation dependent on PARKIN and MUL1. Rojansky R, Cha MY, Chan DC. Elife. 2016 Nov 17;5. pii: e17896. doi: 10.7554/eLife.17896. 10.7554/eLife.17896 PubMed 27852436