pBABEpuro mTurquoise2 LC3B
(Plasmid #78518)

• Purpose: Fluorescent reporter for LC3B visualization
• Depositing Lab: David Chan
• Publication: Rojansky et al Elife. 2016 Nov 17;5. pii: e17896. doi: 10.7554/eLife.17896.

Backbone

• Vector backbone: pBABE puro
• Backbone size w/o insert (bp): 5153
• Total vector size (bp): 6272
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: LC3B
• Species: H. sapiens (human)
• Insert Size (bp): 1119
• Entrez Gene: MAP1LC3B (a.k.a. ATG8F, LC3B, MAP1A/1BLC3, MAP1LC3B-a)
• Promoter: retroviral
• Tag / Fusion Protein:
  • mTurquoise2 (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (unknown if destroyed)
• 3′ cloning site: SalI (unknown if destroyed)
• 5′ sequencing primer: TTTATCCAGCCCTCACTCCTTCTCTAGGC
• 3′ sequencing primer: CCTGGGGACTTTCCACACCCTAAC

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry