GST-PS-Grb2 1-154 (XSB382)
(Plasmid
#78510)
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PurposeConstruct for expressing Grb2 lacking the C-terminal SH3 domain in E. Coli
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 78510 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGEX-6P-1
- Total vector size (bp) 5400
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGrb2 1-154
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SpeciesH. sapiens (human)
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Entrez GeneGRB2 (a.k.a. ASH, EGFRBP-GRB2, Grb3-3, MST084, MSTP084, NCKAP2)
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Tag
/ Fusion Protein
- GST followed by a prescission protease claevage site (N terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
GST-PS-Grb2 1-154 (XSB382) was a gift from Ron Vale (Addgene plasmid # 78510 ; http://n2t.net/addgene:78510 ; RRID:Addgene_78510) -
For your References section:
Phase separation of signaling molecules promotes T cell receptor signal transduction. Su X, Ditlev JA, Hui E, Xing W, Banjade S, Okrut J, King DS, Taunton J, Rosen MK, Vale RD. Science. 2016 Apr 29;352(6285):595-9. doi: 10.1126/science.aad9964. Epub 2016 Apr 7. 10.1126/science.aad9964 PubMed 27056844