pJQ200uc1
(Plasmid #78502)

• Purpose: (Empty Backbone) Suicide vector for gene replacement, modified MCS to maximise utility of insert restriction sites.
• Depositing Lab: Michael Hynes
• Publication: Quandt et al Gene. 1993 May 15;127(1):15-21.

Backbone

• Vector backbone: pJQ200
• Backbone manufacturer: Jurgen Quandt
• Backbone size (bp): 4700
• Modifications to backbone: Added multiple cloning site with synthetic polylinker to allow use of restriction sites within cloned fragments.
• Vector type: Suicide vector for gene replacement
• Promoter: none
• Tag / Fusion Protein:
  • none

Growth in Bacteria

• Bacterial Resistance(s): Gentamicin, 10 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: none
• 3′ sequencing primer: none

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Construct is from our lab.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The new synthetic polylinker (SmaI site flanked by NotI) allows access to restriction sites within a cloned fragment to allow insertion of cassettes etc. Blue/White selection works.