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Addgene

pJQ200mp19
(Plasmid #78501)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 78501 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pJQ200
  • Backbone manufacturer
    Jurgen Quandt
  • Backbone size (bp) 4700
  • Modifications to backbone
    polylinker and lacZ alpha from pUC19 added to pJQ200 as partial PvuI digest.
  • Vector type
    Suicide vector for gene replacement
  • Promoter none

Growth in Bacteria

  • Bacterial Resistance(s)
    Gentamicin, 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Construct is from our lab.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Allows different restriction cloning strategies to be used than pJQ200SK. Since full length lacZ alpha is present, blue white selection works faster than for pJQ200SK

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJQ200mp19 was a gift from Michael Hynes (Addgene plasmid # 78501 ; http://n2t.net/addgene:78501 ; RRID:Addgene_78501)

  • For your References section:

    Versatile suicide vectors which allow direct selection for gene replacement in gram-negative bacteria. Quandt J, Hynes MF. Gene. 1993 May 15;127(1):15-21. 0378-1119(93)90611-6 [pii] PubMed 8486283
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