pJQ200mp18
(Plasmid #78500)

• Purpose: (Empty Backbone) Suicide vector for gene replacement
• Depositing Lab: Michael Hynes
• Publication: Quandt et al Gene. 1993 May 15;127(1):15-21.

Backbone

• Vector backbone: pJQ200
• Backbone manufacturer: Jurgen Quandt
• Backbone size (bp): 4700
• Modifications to backbone: polylinker and lacZ alpha from pUC18 added to pJQ200 as partial PvuI digest.
• Vector type: Suicide vector for gene replacement
• Promoter: none

Growth in Bacteria

• Bacterial Resistance(s): Gentamicin, 10 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: none
• 3′ sequencing primer: none

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Construct is from our lab.
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Allows different restriction cloning strategies to be used than pJQ200SK. Since full length lacZ alpha is present, blue white selection works faster than for pJQ200SK