pJQ210SK
(Plasmid
#78499)
-
Purpose(Empty Backbone) Cosmid version of suicide vector pJQ200SK. Suicide vector
-
Depositing Lab
-
Publication
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 78499 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepJQ200
-
Backbone manufacturerJurgen Quandt
- Backbone size (bp) 4700
-
Modifications to backboneAdded mutliple cloning site and cos site for cosmid cloning.
-
Vector typesuicide vector
- Promoter non
-
Tag
/ Fusion Protein
- none
Growth in Bacteria
-
Bacterial Resistance(s)Gentamicin, 10 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer none
- 3′ sequencing primer none (Common Sequencing Primers)
Resource Information
-
A portion of this plasmid was derived from a plasmid made byPlasmid is our construct. See published paper for cloning methods and component parts.
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Cosmid version of suicide vector for gene replacement. Same as pJQ200SK except for addition of cos site.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pJQ210SK was a gift from Michael Hynes (Addgene plasmid # 78499 ; http://n2t.net/addgene:78499 ; RRID:Addgene_78499) -
For your References section:
Versatile suicide vectors which allow direct selection for gene replacement in gram-negative bacteria. Quandt J, Hynes MF. Gene. 1993 May 15;127(1):15-21. 0378-1119(93)90611-6 [pii] PubMed 8486283