pJQ200KS
(Plasmid
#78498)
-
Purpose(Empty Backbone) Suicide vector
-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 78498 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepJQ200
- Backbone size (bp) 4922
-
Modifications to backboneadded multiple cloning site and partial lacZ alpha gene as PvuII fragment cloned into the SmaI site of pJQ200
-
Vector typeSuicide plasmid
-
Selectable markersGentamicin
Growth in Bacteria
-
Bacterial Resistance(s)Gentamicin, 10 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer T7
- 3′ sequencing primer T3 (Common Sequencing Primers)
Resource Information
-
A portion of this plasmid was derived from a plasmid made byWe constructed this plasmid in the lab
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This is a suicide vector for gene replacement. It is identical to pJQ200SK except that the multiple cloning site is from pBluescriptKS instead of from pBluescriptSK
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pJQ200KS was a gift from Michael Hynes (Addgene plasmid # 78498 ; http://n2t.net/addgene:78498 ; RRID:Addgene_78498) -
For your References section:
Versatile suicide vectors which allow direct selection for gene replacement in gram-negative bacteria. Quandt J, Hynes MF. Gene. 1993 May 15;127(1):15-21. 0378-1119(93)90611-6 [pii] PubMed 8486283
