pJQ200SK
(Plasmid #78497)

• Purpose: (Empty Backbone) Suicide vector for gene replacement
• Depositing Lab: Michael Hynes
• Publication: Quandt et al Gene. 1993 May 15;127(1):15-21.

Backbone

• Vector backbone: pJQ200
• Backbone size (bp): 4922
• Modifications to backbone: added multiple cloning site and partial lacZ alpha gene as PvuII fragment cloned into the SmaI site of pJQ200
• Vector type: Suicide plasmid

Growth in Bacteria

• Bacterial Resistance(s): Gentamicin, 10 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: T7
• 3′ sequencing primer: T3

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This is the most popular version of the pJQ200 series of vectors. The partial lacZ alpha gene does give blue/white selection, but colonies are only pale blue after 24 hours. Blue colour is clear after 36 hrs. or by using higher x-gal concentration.