pJQ200
(Plasmid
#78496)
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Purpose(Empty Backbone) Base vector for construction of series of suicide plasmids for gene replacement in Proteobacteria
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 78496 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneP15A origin vector pACYC184Gm
- Backbone size (bp) 6500
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Modifications to backboneAdded OriT, sacB gene etc in multiple steps.
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Vector typeSuicide vector
Growth in Bacteria
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Bacterial Resistance(s)Gentamicin, 10 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Base vector for suicide vectors pJQ200SK, pJQ200KS, pJQ200mp18, pJQ200mp19 which have been quite widely used for allelic replacement in rhizobia and other bacteria. sacB gene provides basis for selection of double recombinants.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJQ200 was a gift from Michael Hynes (Addgene plasmid # 78496 ; http://n2t.net/addgene:78496 ; RRID:Addgene_78496) -
For your References section:
Versatile suicide vectors which allow direct selection for gene replacement in gram-negative bacteria. Quandt J, Hynes MF. Gene. 1993 May 15;127(1):15-21. 0378-1119(93)90611-6 [pii] PubMed 8486283
Map uploaded by the depositor.
