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Addgene

pLC13
(Plasmid #78494)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 78494 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBAD18
  • Total vector size (bp) 5120
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Mu-gam
  • Entrez Gene
    Mup10 (a.k.a. Mup10)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    pBAD18 (Guzman, 1995, J. Bacteriol)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The Mu-gam insert within this plasmid contains an L126R mutation. The depositor states that this mutation does not affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLC13 was a gift from David Bikard (Addgene plasmid # 78494 ; http://n2t.net/addgene:78494 ; RRID:Addgene_78494)

  • For your References section:

    Consequences of Cas9 cleavage in the chromosome of Escherichia coli. Cui L, Bikard D. Nucleic Acids Res. 2016 Apr 8. pii: gkw223. 10.1093/nar/gkw223 PubMed 27060147
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