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PurposeExpression of GFP under the sulA promoter.
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 78493 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepZA31-Luc
- Total vector size (bp) 2919
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameGFPmut2
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SpeciesE. coli
Resource Information
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A portion of this plasmid was derived from a plasmid made bypZA31 (Lutz, 1997, NAR) and GFPmut2 (Cormack, 1996, Gene)
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pZA31-sulA-GFP was a gift from David Bikard (Addgene plasmid # 78493 ; http://n2t.net/addgene:78493 ; RRID:Addgene_78493) -
For your References section:
Consequences of Cas9 cleavage in the chromosome of Escherichia coli. Cui L, Bikard D. Nucleic Acids Res. 2016 Apr 8. pii: gkw223. 10.1093/nar/gkw223 PubMed 27060147