pCRRNAcos
(Plasmid #78492)

• Purpose: Transcription of Streptococcus pyogenes crRNA and tracrRNA under the wildtype crRNA promoter.
• Depositing Lab: David Bikard
• Publication: Cui et al Nucleic Acids Res. 2016 Apr 8. pii: gkw223.

Backbone

• Vector backbone: pUC19
• Total vector size (bp): 2650
• Modifications to backbone: changed to be Kanamycin resistant
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: tracrRNA
• Species: E. coli

Resource Information

• A portion of this plasmid was derived from a plasmid made by: pCas9 and pCRISPR (Jiang, 2013, Nat. Biotech.)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry