AAA
(Plasmid
#78487)
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Purpose2135nt RNA encoded by chimaeric gene cloned in a pSP65A vector that included about 65 dT residues for transcription of a poly(A) tail
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 78487 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepSP65A
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)TOP10 F'
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Growth instructionsMUST be grown in TOP10 F' to maintain poly A
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameCAN1-LUC
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SpeciesS. cerevisiae (budding yeast)
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MutationCAN1 contains no stop codon
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Note: the poly A tends to undergo recombination to a shorter tract. It is important to sequence a few isolates from a transformation or prep to ensure integrity. Please see Fig 1 of associated publication for general schematic
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
AAA was a gift from Allan Jacobson (Addgene plasmid # 78487 ; http://n2t.net/addgene:78487 ; RRID:Addgene_78487) -
For your References section:
Translation factors promote the formation of two states of the closed-loop mRNP. Amrani N, Ghosh S, Mangus DA, Jacobson A. Nature. 2008 Jun 26;453(7199):1276-80. doi: 10.1038/nature06974. Epub 2008 May 21. 10.1038/nature06974 PubMed 18496529