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Addgene

miniUAA3
(Plasmid #78482)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 78482 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSP65A
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    TOP10 F'
  • Growth instructions
    MUST be grown in TOP10 F' to maintain poly A
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    CAN1-LUC
  • Species
    S. cerevisiae (budding yeast)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Note: the poly A tends to undergo recombination to a shorter tract. It is important to sequence a few isolates from a transformation or prep to ensure integrity. Please see Fig 1 of associated publication for general schematic

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    miniUAA3 was a gift from Allan Jacobson (Addgene plasmid # 78482 ; http://n2t.net/addgene:78482 ; RRID:Addgene_78482)

  • For your References section:

    Translation factors promote the formation of two states of the closed-loop mRNP. Amrani N, Ghosh S, Mangus DA, Jacobson A. Nature. 2008 Jun 26;453(7199):1276-80. doi: 10.1038/nature06974. Epub 2008 May 21. 10.1038/nature06974 PubMed 18496529
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