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PurposeBroad range bacterial fluorescence labeling
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 78478 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUCP30T
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Gentamicin, 10 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameE2-Crimson
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SpeciesDiscosoma sp.
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
There is an ER retention signal at the C-terminus of the E2-Crimson ORF. This sequence is based on the template used to generate this vector. It is not predicted to impact expression or function in bacteria.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pUCP30T-E2Crimson was a gift from Mariette Barbier (Addgene plasmid # 78478 ; http://n2t.net/addgene:78478 ; RRID:Addgene_78478) -
For your References section:
Rainbow Vectors for Broad-Range Bacterial Fluorescence Labeling. Barbier M, Damron FH. PLoS One. 2016 Mar 3;11(3):e0146827. doi: 10.1371/journal.pone.0146827. eCollection 2016. 10.1371/journal.pone.0146827 PubMed 26937640