Sumo3 PolQM1
(Plasmid #78462)

• Purpose: Inducible expression in E. coli
• Depositing Labs: Sylvie Doublie, Susan Wallace
• Publication: Hogg et al J Mol Biol. 2011 Jan 21;405(3):642-52. doi: 10.1016/j.jmb.2010.10.041. Epub 2010 Nov 2.

Backbone

• Vector backbone: pE-SUMO3 fusion vector
• Backbone manufacturer: Life Sensors
• Backbone size w/o insert (bp): 5743
• Vector type: Bacterial Expression
• Selectable markers: none

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Polymerase theta
• Alt name: POLQ
• Species: H. sapiens (human)
• Mutation: delta 1-1791; Q2513R (please see depositor comments below)
• GenBank ID: 10721
• Entrez Gene: POLQ (a.k.a. PRO0327)
• Promoter: T7
• Tags / Fusion Proteins:
  • His (N terminal on backbone)
  • SUMO3 (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BsaI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7term

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Original full length POLQ gene came from Rick Wood (then at University of Pittsburgh, now at MD Anderson).
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Has SUMO3 protease cleavage site. Please note that mutation Q2513R in POLQ was found during Addgene's quality control. The depositor noted that Q2513R is an amino acid variant representing a common human snp (T instead of C) rs1381057. This mutation does NOT affect plasmid function.