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Sumo3 PolQM1 deltains1
(Plasmid #78461)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 78461 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pE-SUMO3 fusion vector
  • Backbone manufacturer
    Life Sensors
  • Backbone size w/o insert (bp) 5743
  • Vector type
    Bacterial Expression
  • Selectable markers
    none

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Polymerase theta
  • Alt name
    POLQ
  • Species
    H. sapiens (human)
  • Mutation
    delta 1-1791; delta 2149-2170; Q2513R (please see depositor comment below)
  • GenBank ID
    10721
  • Entrez Gene
    POLQ (a.k.a. PRO0327)
  • Promoter T7
  • Tags / Fusion Proteins
    • His (N terminal on backbone)
    • SUMO3 (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsaI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7term
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Original full length POLQ gene came from Rick Wood (then at University of Pittsburgh, now at MD Anderson).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Has SUMO3 protease cleavage site. Please note that mutation Q2513R in POLQ was found during Addgene's quality control. The depositor noted that Q2513R is an amino acid variant representing a common human snp (T instead of C) rs1381057. This mutation does NOT affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Sumo3 PolQM1 deltains1 was a gift from Sylvie Doublie & Susan Wallace (Addgene plasmid # 78461 ; http://n2t.net/addgene:78461 ; RRID:Addgene_78461)

  • For your References section:

    Lesion bypass activity of DNA polymerase theta (POLQ) is an intrinsic property of the pol domain and depends on unique sequence inserts. Hogg M, Seki M, Wood RD, Doublie S, Wallace SS. J Mol Biol. 2011 Jan 21;405(3):642-52. doi: 10.1016/j.jmb.2010.10.041. Epub 2010 Nov 2. 10.1016/j.jmb.2010.10.041 PubMed 21050863
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