HisMBP CFlm25
(Plasmid #78458)

• Purpose: Inducible expression in E. coli
• Depositing Lab: Sylvie Doublie
• Publication: Coseno et al Nucleic Acids Res. 2008 Jun;36(10):3474-83. doi: 10.1093/nar/gkn079. Epub 2008 Apr 29.

Backbone

• Vector backbone: pDEST-HisMBP fusion vector
• Backbone manufacturer: David Waugh, National Cancer Institute, NIH
• Backbone size w/o insert (bp): 8282
• Total vector size (bp): 8963
• Vector type: Bacterial Expression
• Selectable markers: none

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Human Cleavage Factor lm 25 kDa subunit
• Alt name: CPSF5
• Alt name: NUDT21
• Species: H. sapiens (human)
• Insert Size (bp): 681
• GenBank ID: 11051
• Entrez Gene: NUDT21 (a.k.a. CFIM25, CPSF5)
• Promoter: tac
• Tags / Fusion Proteins:
  • His (N terminal on backbone)
  • MBP (N terminal on backbone)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: MBP-F
• 3′ sequencing primer: M13-F20

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Original CFlm25 gene came from Greg Gilmartin (University of Vermont).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Has a TEV cleavage site.