pV92.2
(Plasmid #78409)

• Purpose: pKT230 plasmid engineered with the RTCas1v2-GFP cassette (RT-Cas1, Cas2, GFP) driven by the MMB-1 16S rRNA promoter, the CRISPR03 array driven by its native leader, and the tmRNA::td intron construct
• Depositing Lab: Andrew Fire
• Publication: Silas et al Science. 2016 Feb 26;351(6276):aad4234. doi: 10.1126/science.aad4234.

Backbone

• Vector backbone: pKT230
• Backbone size w/o insert (bp): 12000
• Total vector size (bp): 16146

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert 1

• Gene/Insert name: RT-Cas1
• Species: Marinomonas mediterranea MMB-1
• Insert Size (bp): 2874
• GenBank ID: ADZ89953.1

Gene/Insert 2

• Gene/Insert name: Cas2
• Species: Marinomonas mediterranea MMB-1
• Insert Size (bp): 279
• GenBank ID: ADZ89952.1

Resource Information

• Supplemental Documents:
  • Annotated Genbank file

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry