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Addgene

pNX3b-PK12
(Plasmid #78364)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 78364 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFA6a-hygMX6
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    12xPK
  • Alt name
    V5

Gene/Insert 2

  • Gene/Insert name
    hygMX6
  • Alt name
    hphMX6
  • Species
    S. cerevisiae (budding yeast), S. pombe (fission yeast)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pNX3b-PK12 was a gift from Kazunori Tomita (Addgene plasmid # 78364 ; http://n2t.net/addgene:78364 ; RRID:Addgene_78364)

  • For your References section:

    Sequential and counter-selectable cassettes for fission yeast. Amelina H, Moiseeva V, Collopy LC, Pearson SR, Armstrong CA, Tomita K. BMC Biotechnol. 2016 Nov 8;16(1):76. 10.1186/s12896-016-0307-4 [pii] PubMed 27825338
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