FLAG-hSRF (#482)
(Plasmid #78343)

• Purpose: Overexpression of human SRF ORF with a N-terminal FLAG tag
• Depositing Labs: Matthew Alexander, Louis Kunkel
• Publication: Alexander lab Muscle Factors (unpublished)

Backbone

• Vector backbone: pCMV-Tag2b
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 4300
• Total vector size (bp): 5824
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SRF
• Alt name: serum response factor
• Species: H. sapiens (human)
• Insert Size (bp): 1524
• GenBank ID: NM_003131.3
• Entrez Gene: SRF (a.k.a. MCM1)
• Promoter: CMV
• Tag / Fusion Protein:
  • FLAG (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamH1 (not destroyed)
• 3′ cloning site: EcoR1 (not destroyed)
• 5′ sequencing primer: T3 promoter
• 3′ sequencing primer: T7 promoter

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please contact Dr. Alexander if you have any questions.