hMYOG-3xHA-IRES-hrGFP (#324)
(Plasmid #78341)

• Purpose: Overexpression of human MYOG ORF with a 3xHA C-terminal tag and an IRES-hrGFP reporter
• Depositing Labs: Matthew Alexander, Louis Kunkel
• Publication: Alexander lab Muscle Factors (unpublished)

Backbone

• Vector backbone: pIRES-2a-hrGFP
• Backbone manufacturer: Agilent Technologies
• Backbone size w/o insert (bp): 5000
• Total vector size (bp): 5750
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: MYOG
• Alt name: myogenin
• Alt name: myogenic factor 4
• Species: H. sapiens (human)
• Insert Size (bp): 672
• GenBank ID: NM_002479.5
• Entrez Gene: MYOG (a.k.a. MYF4, bHLHc3, myf-4)
• Promoter: CMV
• Tags / Fusion Proteins:
  • 3x HA tag (C terminal on backbone)
  • IRES-hrGFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Not1 (not destroyed)
• 3′ cloning site: Xho1 (not destroyed)
• 5′ sequencing primer: CAAGGATGACGACGATAAG
• 3′ sequencing primer: CTATTAAGCGTAGTCAGGTACATC

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Contains a 2 amino acid deletion. Does not affect function. Please contact Dr. Alexander if you have any questions.