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PurposeOverexpression of human PAX3 ORF with a N-terminal FLAG tag
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Depositing Labs
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 78335 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCMV-Tag2b
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Backbone manufacturerStratagene
- Backbone size w/o insert (bp) 4300
- Total vector size (bp) 5800
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePAX3
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Alt namepaired box 3
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SpeciesH. sapiens (human)
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Insert Size (bp)1500
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GenBank IDBC101301.2
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Entrez GenePAX3 (a.k.a. CDHS, HUP2, PAX-3, WS1, WS3)
- Promoter CMV
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Tag
/ Fusion Protein
- FLAG (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamH1 (not destroyed)
- 3′ cloning site EcoR1 (not destroyed)
- 5′ sequencing primer T3 promoter
- 3′ sequencing primer T7 promoter (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please contact Dr. Alexander if you have any questions.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
FLAG-hPAX3 (#395) was a gift from Matthew Alexander & Louis Kunkel (Addgene plasmid # 78335 ; http://n2t.net/addgene:78335 ; RRID:Addgene_78335)
Map uploaded by the depositor.
