pINDUCER20-FLAG-hMYF5 (#801)
(Plasmid #78334)

• Purpose: Doxycycline-inducible lentiviral overexpression of human MYF5 ORF with a FLAG tag
• Depositing Labs: Matthew Alexander, Louis Kunkel
• Publication: Alexander lab Muscle Factors (unpublished)

Backbone

• Vector backbone: pInducer20 (Addgene plasmid #44012)
• Backbone manufacturer: Stephen Elledge
• Backbone size w/o insert (bp): 11791
• Total vector size (bp): 12591
• Vector type: Lentiviral
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: MYF5
• Alt name: Myogenic Factor 5
• Species: H. sapiens (human)
• Insert Size (bp): 765
• GenBank ID: NM_005593.2
• Entrez Gene: MYF5 (a.k.a. EORVA, bHLHc2)
• Promoter: TRE2
• Tag / Fusion Protein:
  • FLAG (N terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: CAAGGATGACGACGATAAG

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please contact Dr. Alexander if you have any questions.