pINDUCER20-hMYOD1-3xHA (#730)
(Plasmid #78328)

• Purpose: Doxycycline-inducible lentiviral overexpression of human MYOD1 ORF with a 3xHA tag
• Depositing Labs: Matthew Alexander, Louis Kunkel
• Publication: Alexander lab Muscle Factors (unpublished)

Backbone

• Vector backbone: pInducer20 (Addgene plasmid #44012)
• Backbone manufacturer: Stephen Elledge
• Backbone size w/o insert (bp): 11791
• Total vector size (bp): 12791
• Vector type: Lentiviral
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: MYOD1
• Alt name: Myogenic differentiation 1
• Species: H. sapiens (human)
• Insert Size (bp): 1000
• GenBank ID: NM_002478.4
• Entrez Gene: MYOD1 (a.k.a. CMYP17, MYF3, MYOD, MYODRIF, PUM, bHLHc1)
• Promoter: TRE2
• Tag / Fusion Protein:
  • 3x HA tag (C terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: CAAGGATGACGACGATAAG

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Validated in conversion of fibroblasts to myoblasts. Please contact Dr. Alexander if you have any questions.