pLenCMV-PMMars1CyGFP
(Plasmid #78315)

• Purpose: Lentiviral transfer vector encoding Mars1Cy-GFP membrane-spanning protein
• Depositing Lab: Alan Waggoner
• Publication: Zhang et al J Clin Invest. 2015 Oct 1;125(10):3915-27. doi: 10.1172/JCI81086. Epub 2015 Sep 8.

Backbone

• Vector backbone: pLenti
• Backbone size w/o insert (bp): 9786
• Total vector size (bp): 11454
• Modifications to backbone: Replaced CaMKII promoter with CMV
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Mars1Cy-TMD-GFP
• Alt name: dL9.5-TMD-GFP
• Insert Size (bp): 1668
• Promoter: CMV
• Tag / Fusion Protein:
  • HA epitope (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AgeI (not destroyed)
• 3′ cloning site: BsrGI (not destroyed)
• 5′ sequencing primer: GCgctaccggtcgccaccATG

Resource Information

• Supplemental Documents:
  • Sequence of pLenCMV-PMMars1CyGFP from APE.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry