HpMscS
(Plasmid #78310)

• Purpose: H. pylori MscS with N-Term 6x Hisitidine Tag and C-Term FLAG Tag
• Depositing Lab: Douglas Rees
• Publication: Lai et al Protein Sci. 2013 Apr;22(4):502-9. doi: 10.1002/pro.2222. Epub 2013 Feb 21.

Backbone

• Vector backbone: pET28b
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5300
• Total vector size (bp): 6130
• Modifications to backbone: Changed C-Term His to FLAG Tag
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Mechanosensitive channel of small conductance
• Alt name: MscS
• Alt name: H. pylori MscS
• Species: Helicobactor pylori
• Insert Size (bp): 823
• Promoter: T7 Promoter
• Tags / Fusion Proteins:
  • 6x His Tag (N terminal on backbone)
  • FLAG Tag (C terminal on backbone)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: T7 Forward
• 3′ sequencing primer: T7 Terminator

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry