NaAtm1
(Plasmid
#78308)
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PurposeAtm1 type ABC Exporter
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 78308 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepJL-H6
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Backbone manufacturerCustom Plasmid created in Rees Lab
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth TemperatureRoom Temperature
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameAtm1 type ABC Exporter
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Alt nameAtm1
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SpeciesN. aromaticivorans
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Insert Size (bp)1827
- Promoter T7 Promoter
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Tag
/ Fusion Protein
- 6x Histidine Tag (C terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer T7 Forward
- 3′ sequencing primer T7 Reverse (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
NaAtm1 was a gift from Douglas Rees (Addgene plasmid # 78308 ; http://n2t.net/addgene:78308 ; RRID:Addgene_78308) -
For your References section:
Structural basis for heavy metal detoxification by an Atm1-type ABC exporter. Lee JY, Yang JG, Zhitnitsky D, Lewinson O, Rees DC. Science. 2014 Mar 7;343(6175):1133-6. doi: 10.1126/science.1246489. 10.1126/science.1246489 PubMed 24604198