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Addgene

Lf-MetQ
(Plasmid #78305)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 78305 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET21b
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5367
  • Total vector size (bp) 6180
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Methionine Binding Protein
  • Alt name
    MetQ
  • Insert Size (bp)
    813
  • Entrez Gene
    metQ (a.k.a. b0197, ECK0197, metD, yaeC)
  • Tag / Fusion Protein
    • 6x Histidine Tag (C terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Lf-MetQ was a gift from Douglas Rees (Addgene plasmid # 78305 ; http://n2t.net/addgene:78305 ; RRID:Addgene_78305)

  • For your References section:

    The contribution of methionine to the stability of the Escherichia coli MetNIQ ABC transporter-substrate binding protein complex. Nguyen PT, Li QW, Kadaba NS, Lai JY, Yang JG, Rees DC. Biol Chem. 2015 Sep;396(9-10):1127-34. doi: 10.1515/hsz-2015-0131. /j/bchm.just-accepted/hsz-2015-0131/hsz-2015-0131.xml [pii] PubMed 25803078
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