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Addgene

FAT FAK biosensor
(Plasmid #78303)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 78303 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FAT FAK biosensor
  • Alt name
    ECFP-SH2 domain of c-Src-substrate-YPet-FAT domain from FAK
  • Species
    Synthetic
  • Insert Size (bp)
    2200
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FAT FAK biosensor was a gift from Yingxiao Wang (Addgene plasmid # 78303 ; http://n2t.net/addgene:78303 ; RRID:Addgene_78303)

  • For your References section:

    Distinct biophysical mechanisms of focal adhesion kinase mechanoactivation by different extracellular matrix proteins. Seong J, Tajik A, Sun J, Guan JL, Humphries MJ, Craig SE, Shekaran A, Garcia AJ, Lu S, Lin MZ, Wang N, Wang Y. Proc Natl Acad Sci U S A. 2013 Nov 26;110(48):19372-7. doi: 10.1073/pnas.1307405110. Epub 2013 Nov 12. 10.1073/pnas.1307405110 PubMed 24222685
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