Kras-Src FRET biosensor
(Plasmid #78302)

• Purpose: FRET biosensor. To monitor Src kinase activity in mammalian cells by FRET
• Depositing Lab: Yingxiao Wang
• Publication: Ouyang et al Proc Natl Acad Sci U S A. 2008 Sep 23;105(38):14353-8. doi: 10.1073/pnas.0807537105. Epub 2008 Sep 17.

Backbone

• Vector backbone: pcDNA3.1
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5400
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Src biosensor
• Alt name: ECFP-SH2-linker-substrate-YPet-KRasTag
• Species: Synthetic
• Insert Size (bp): 1800
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Resource Information

• Articles Citing this Plasmid:
  • 6 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry