pCAG-cofilin-S3A
(Plasmid #78296)

• Purpose: Mammalian expression of mouse cofilin S3A (nonphosphorylatable form) fused to EGFP
• Depositing Lab: Michael Frotscher
• Publication: Chai et al Development. 2016 Mar 15;143(6):1029-40. doi: 10.1242/dev.134163. Epub 2016 Feb 18.

Backbone

• Vector backbone: pCAG-MCS
• Backbone size w/o insert (bp): 5197
• Total vector size (bp): 6404
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: cofilin 1
• Species: M. musculus (mouse)
• Insert Size (bp): 1264
• Mutation: S3A
• Entrez Gene: Cfl1 (a.k.a. Cof)
• Promoter: CAG
• Tag / Fusion Protein:
  • EGFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: BglII (not destroyed)
• 5′ sequencing primer: pCAG-F
• 3′ sequencing primer: EGFP-N

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid was made by Wei Zhang.