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pCAG-cofilin WT
(Plasmid #78295)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 78295 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCAG-MCS
  • Backbone size w/o insert (bp) 5197
  • Total vector size (bp) 6404
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    cofilin 1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1264
  • Entrez Gene
    Cfl1 (a.k.a. Cof)
  • Promoter CAG
  • Tag / Fusion Protein
    • EGFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site BglII (not destroyed)
  • 5′ sequencing primer pCAG-F
  • 3′ sequencing primer EGFP-N
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid was made by Wei Zhang.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAG-cofilin WT was a gift from Michael Frotscher (Addgene plasmid # 78295 ; http://n2t.net/addgene:78295 ; RRID:Addgene_78295)

  • For your References section:

    Reelin and cofilin cooperate during the migration of cortical neurons: a quantitative morphological analysis. Chai X, Zhao S, Fan L, Zhang W, Lu X, Shao H, Wang S, Song L, Failla AV, Zobiak B, Mannherz HG, Frotscher M. Development. 2016 Mar 15;143(6):1029-40. doi: 10.1242/dev.134163. Epub 2016 Feb 18. 10.1242/dev.134163 PubMed 26893343
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