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Addgene

pTfdc1Sc
(Plasmid #78287)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 78287 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTrc99A
  • Backbone size w/o insert (bp) 4176
  • Total vector size (bp) 5695
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FDC1
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    1512
  • Entrez Gene
    FDC1 (a.k.a. YDR539W)
  • Promoter trc

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer Jasanoff-Rev (5'-gctcatgtttgacagcttatcatc-3')
  • 3′ sequencing primer pCR8_F1 (5'-ttgatgcctggcagttccct-3')
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTfdc1Sc was a gift from David Nielsen (Addgene plasmid # 78287 ; http://n2t.net/addgene:78287 ; RRID:Addgene_78287)

  • For your References section:

    Styrene biosynthesis from glucose by engineered E. coli. McKenna R, Nielsen DR. Metab Eng. 2011 Sep;13(5):544-54. doi: 10.1016/j.ymben.2011.06.005. Epub 2011 Jun 23. 10.1016/j.ymben.2011.06.005 PubMed 21722749
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