J23114-mRFP1-331Bb
(Plasmid
#78279)
-
PurposemRFP1 behind constitutive promoter J23114 in pSEVA331Bb backbone
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 78279 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepSEVA331Bb
- Backbone size w/o insert (bp) 2893
Growth in Bacteria
-
Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert nameJ23114-mRFP1
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
J23114-mRFP1-331Bb was a gift from Tom Ellis (Addgene plasmid # 78279 ; http://n2t.net/addgene:78279 ; RRID:Addgene_78279) -
For your References section:
Engineering control of bacterial cellulose production using a genetic toolkit and a new cellulose-producing strain. Florea M, Hagemann H, Santosa G, Abbott J, Micklem CN, Spencer-Milnes X, de Arroyo Garcia L, Paschou D, Lazenbatt C, Kong D, Chughtai H, Jensen K, Freemont PS, Kitney R, Reeve B, Ellis T. Proc Natl Acad Sci U S A. 2016 Jun 14;113(24):E3431-40. doi: 10.1073/pnas.1522985113. Epub 2016 May 31. 10.1073/pnas.1522985113 PubMed 27247386