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Addgene

pCIG3 Rab5CA
(Plasmid #78268)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 78268 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCIG3
  • Backbone manufacturer
    Goodrum Lab, Addgene plasmid
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    RAB5A
  • Species
    H. sapiens (human)
  • Mutation
    Q79L
  • Entrez Gene
    RAB5A (a.k.a. RAB5)
  • Promoter CMV, Lentiviral LTR
  • Tags / Fusion Proteins
    • mCherry (N terminal on insert)
    • IRES-EGFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer mCherry-F
  • 3′ sequencing primer pCDH-rev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Subcloned from mCherry-Rab5CA(Q79L) from Sergio Grinstein (Addgene plasmid # 35138)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCIG3 Rab5CA was a gift from Felicia Goodrum (Addgene plasmid # 78268 ; http://n2t.net/addgene:78268 ; RRID:Addgene_78268)
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