pCIG3 (pCMV-IRES-GFP version 3)
(Plasmid #78264)

• Purpose: (Empty Backbone) Lentiviral empty cloning vector containing IRES and GFP
• Depositing Lab: Felicia Goodrum
• Publication: Caviness et al J Virol. 2014 Dec;88(24):14412-25. doi: 10.1128/JVI.02711-14. Epub 2014 Oct 8.

Backbone

• Vector backbone: Derived from pCIG1
• Backbone manufacturer: Goodrum lab, Addgene plasmid 78265
• Backbone size (bp): 7624
• Vector type: Mammalian Expression, Lentiviral
• Promoter: CMV, lentiviral LTR
• Tag / Fusion Protein:
  • IRES-EGFP (C terminal on backbone)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Low Copy

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: pCDH-Rev

Resource Information

• Supplemental Documents:
  • genbank file
• Articles Citing this Plasmid:
  • 16 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This vector differs from pCIG1 in the following manners:
- Different IRES (derived from Clontech pIRES-GFP), which is easier to mutagenize, PCR, and sanger sequence.
- Expanded MCS, derived from linker oligo cloning.
- No longer contains an unannotated second XbaI site. XbaI site in MCS can be used for cloning.

Information for Cloning Grade DNA (Catalog # 78264-DNA.cg) ( Back to top)

Purpose

Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.

Delivery

• Amount: 2 µg
• Guaranteed Concentration: 100 ng/µl +/- 5 ng/µl
• Pricing: $105 USD
• Storage: DNA can be stored at 4℃ (short term) or -20℃ (long term).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Addgene has verified this plasmid using Next Generation Sequencing. Results are available here