pTol2p2A--3mnx1:loxP-mTagBFP2-stop-loxP-mCherry-NS-MCS
(Plasmid #78251)

• Purpose: Can be used to subclone in tagged gene of interest into inducible motor neuron expression vector.
• Depositing Lab: Nicholas Cole
• Publication: Don et al Zebrafish. 2016 Sep 15.

Backbone

• Vector backbone: pTol2pA2
• Backbone manufacturer: Chien Lab
• Backbone size w/o insert (bp): 4097
• Total vector size (bp): 8906
• Vector type: Zebrafish motor neuron inducible expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: -3mnx1
• Alt name: mnx1, Hb9
• Species: D. rerio (zebrafish)
• Insert Size (bp): 4809
• Entrez Gene: mnx1 (a.k.a. hlxb, hlxb9, zgc:112174)
• Promoter: -3mnx1

Cloning Information for Gene/Insert 1

• Cloning method: Gateway Cloning
• 5′ sequencing primer: Tol2-F [5'-CAATCCTGCAGTGCTGAAAA-3']
• 3′ sequencing primer: pBluescript-SK [5'-tctagaactagtggatc-3']

Gene/Insert 2

• Gene/Insert name: -loxP-mTagBFP2-stop-

Gene/Insert 3

• Gene/Insert name: mCherry-NS-MCS

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that there are some discrepancies between Addgene's quality control sequences and the depositor's sequence. The depositor noted that these discrepancies do NOT affect plasmid function.