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Addgene

pDHJS4 AN-
(Plasmid #78247)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 78247 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDHJS3 AN-
  • Backbone manufacturer
    Addgene #78241
  • Backbone size w/o insert (bp) 3413
  • Modifications to backbone
    inserted attB and attP sites

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    This plasmid requires a helper phagemid to make single stranded DNA. Suggested helper phaemids: M13K07 (available from NEB) or pM13g (available from Andrew Bradbury at Los Alamos National Laboratory- PMID: 17088290). If using M13K07 helper phagemid, transform plasmid DNA in XL2 MRF'. If using pM13g helper phagemid, transform plasmid DNA in DH5alpha.
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    attB
  • Insert Size (bp)
    48

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (destroyed during cloning)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer none
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    attP
  • Insert Size (bp)
    75

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site NdeI (destroyed during cloning)
  • 5′ sequencing primer none
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDHJS4 AN- was a gift from Tao-shih Hsieh (Addgene plasmid # 78247 ; http://n2t.net/addgene:78247 ; RRID:Addgene_78247)

  • For your References section:

    Improved methods for creating migratable Holliday junction substrates. Chen SH, Plank JL, Willcox S, Griffith JD, Hsieh TS. Nucleic Acids Res. 2013 Mar 1;41(5):e60. doi: 10.1093/nar/gks1343. Epub 2012 Dec 28. 10.1093/nar/gks1343 PubMed 23275569
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