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Addgene

pDHJS3 AS+
(Plasmid #78236)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 78236 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBluescript SK+
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 2961
  • Total vector size (bp) 3413
  • Modifications to backbone
    inserted two loxP sites with sequence A in between
  • Vector type
    phagemid

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    This plasmid requires a helper phagemid to make single stranded DNA. Suggested helper phaemids: M13K07 (available from NEB) or pM13g (available from Andrew Bradbury at Los Alamos National Laboratory- PMID: 17088290). If using M13K07 helper phagemid, transform plasmid DNA in XL2 MRF'. If using pM13g helper phagemid, transform plasmid DNA in DH5alpha.
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    loxP
  • Species
    P1
  • Insert Size (bp)
    34

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer none
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    sequence "A"
  • Species
    D. melanogaster (fly)
  • Insert Size (bp)
    300

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer none
    • (Common Sequencing Primers)

Gene/Insert 3

  • Gene/Insert name
    loxP2
  • Species
    P1
  • Insert Size (bp)
    34

Cloning Information for Gene/Insert 3

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site NdeI (not destroyed)
  • 5′ sequencing primer none
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDHJS3 AS+ was a gift from Tao-shih Hsieh (Addgene plasmid # 78236 ; http://n2t.net/addgene:78236 ; RRID:Addgene_78236)

  • For your References section:

    A novel, topologically constrained DNA molecule containing a double Holliday junction: design, synthesis, and initial biochemical characterization. Plank JL, Hsieh TS. J Biol Chem. 2006 Jun 23;281(25):17510-6. Epub 2006 Apr 11. 10.1074/jbc.M602933200 PubMed 16608853
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