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PurposeTranscripts the r_oligo part that includes the HIV reverse transcriptase binding site (HTBS)
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 78235 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepSB3C5
- Backbone size w/o insert (bp) 2729
- Total vector size (bp) 2946
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namer_oligo
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SpeciesSynthetic
- Promoter j23100
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer na
- 3′ sequencing primer na (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pO_72 was a gift from Christopher Voigt (Addgene plasmid # 78235 ; http://n2t.net/addgene:78235 ; RRID:Addgene_78235) -
For your References section:
Genetic encoding of DNA nanostructures and their self-assembly in living bacteria. Elbaz J, Yin P, Voigt CA. Nat Commun. 2016 Apr 19;7:11179. doi: 10.1038/ncomms11179. 10.1038/ncomms11179 PubMed 27091073
