pDBTrp-LexABD-CRY2FL
(Plasmid
#78210)
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PurposeUse with Addgene 40615 for blue light transcription control in yeast
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 78210 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDBTrp
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Backbone manufacturercustom
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Vector typeYeast Expression
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Selectable markersTRP1
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameLexABD-CRY2
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Alt namecry2
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SpeciesA. thaliana (mustard weed)
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Mutationcontains endogenous CRY2 NLS
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Entrez GeneCRY2 (a.k.a. AT1G04400, AT-PHH1, ATCRY2, CRYPTOCHROME 2 APOPROTEIN, F19P19.14, F19P19_14, FHA, PHH1, cryptochrome 2)
- Promoter ADH1
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Tag
/ Fusion Protein
- lexA binding domain (N terminal on insert)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer ADH1 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDBTrp-LexABD-CRY2FL was a gift from Chandra Tucker (Addgene plasmid # 78210 ; http://n2t.net/addgene:78210 ; RRID:Addgene_78210) -
For your References section:
Benchmarking of optical dimerizer systems. Pathak GP, Strickland D, Vrana JD, Tucker CL. ACS Synth Biol. 2014 Nov 21;3(11):832-8. doi: 10.1021/sb500291r. Epub 2014 Nov 5. 10.1021/sb500291r PubMed 25350266