Skip to main content
Addgene

pDBTrp-LexABD-CRY2FL
(Plasmid #78210)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 78210 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pDBTrp
  • Backbone manufacturer
    custom
  • Vector type
    Yeast Expression
  • Selectable markers
    TRP1

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    LexABD-CRY2
  • Alt name
    cry2
  • Species
    A. thaliana (mustard weed)
  • Mutation
    contains endogenous CRY2 NLS
  • Entrez Gene
    CRY2 (a.k.a. AT1G04400, AT-PHH1, ATCRY2, CRYPTOCHROME 2 APOPROTEIN, F19P19.14, F19P19_14, FHA, PHH1, cryptochrome 2)
  • Promoter ADH1
  • Tag / Fusion Protein
    • lexA binding domain (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDBTrp-LexABD-CRY2FL was a gift from Chandra Tucker (Addgene plasmid # 78210 ; http://n2t.net/addgene:78210 ; RRID:Addgene_78210)
  • For your References section:

    Benchmarking of optical dimerizer systems. Pathak GP, Strickland D, Vrana JD, Tucker CL. ACS Synth Biol. 2014 Nov 21;3(11):832-8. doi: 10.1021/sb500291r. Epub 2014 Nov 5. 10.1021/sb500291r PubMed 25350266