CAG::Gdnf
(Plasmid
#78209)
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Purposepiggybac vector for mouse Gdnf expression
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 78209 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepXL-BacII
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Backbone manufacturerLi et al., PMID 15663772
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Vector typeMammalian Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameGdnf
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SpeciesM. musculus (mouse)
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Entrez GeneGdnf (a.k.a. ATF)
- Promoter CAG
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer pCAGGS5 (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Contains:
1) CAG promoter (same as in our tol2 constructs; the promoter includes a sterile 5'exon)
2) mouse Gdnf gene (cloned by us)
3) bovine growth hormone pA site (same as in tol2 constructs)
4) a loxP-flanked hygromycin resistance cassette
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CAG::Gdnf was a gift from Ivo Lieberam (Addgene plasmid # 78209 ; http://n2t.net/addgene:78209 ; RRID:Addgene_78209) -
For your References section:
Optical control of muscle function by transplantation of stem cell-derived motor neurons in mice. Bryson JB, Machado CB, Crossley M, Stevenson D, Bros-Facer V, Burrone J, Greensmith L, Lieberam I. Science. 2014 Apr 4;344(6179):94-7. doi: 10.1126/science.1248523. 10.1126/science.1248523 PubMed 24700859