pJL1703
(Plasmid
#78196)
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Purposeoptogenetic activation of IL2 neuron in worms
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 78196 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepPD96.52
- Backbone size w/o insert (bp) 3684
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Vector typeWorm Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameChR2(gf)
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SpeciesC. elegans (nematode)
- Promoter klp-6
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer M13 reverse
- 3′ sequencing primer pGEX 3 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This plasmid contains 1-51 of Uncharacterized protein CELE_F32A7.6 (NP_493594.1). The depositor states that this should not adversely affect plasmid function.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJL1703 was a gift from Junho Lee (Addgene plasmid # 78196 ; http://n2t.net/addgene:78196 ; RRID:Addgene_78196) -
For your References section:
Nictation, a dispersal behavior of the nematode Caenorhabditis elegans, is regulated by IL2 neurons. Lee H, Choi MK, Lee D, Kim HS, Hwang H, Kim H, Park S, Paik YK, Lee J. Nat Neurosci. 2011 Nov 13;15(1):107-12. doi: 10.1038/nn.2975. 10.1038/nn.2975 PubMed 22081161