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Addgene

pJL1400
(Plasmid #78190)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 78190 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pPD95.77
  • Vector type
    Worm Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    hsp-16.1
  • Species
    C. elegans (nematode)
  • Entrez Gene
    hsp-16.1 (a.k.a. CELE_T27E4.8)
  • Promoter hsp-16.1

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)
  • 5′ sequencing primer M13 reverse
  • 3′ sequencing primer GFP R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJL1400 was a gift from Junho Lee (Addgene plasmid # 78190 ; http://n2t.net/addgene:78190 ; RRID:Addgene_78190)

  • For your References section:

    Hypoxia-inducible Factor-1 (HIF-1)-independent hypoxia response of the small heat shock protein hsp-16.1 gene regulated by chromatin-remodeling factors in the nematode Caenorhabditis elegans. Lee J, Lee J. J Biol Chem. 2013 Jan 18;288(3):1582-9. doi: 10.1074/jbc.M112.401554. Epub 2012 Dec 10. 10.1074/jbc.M112.401554 PubMed 23229554
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