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Addgene

pJL1121
(Plasmid #78188)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 78188 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pPD95.77
  • Vector type
    Worm Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    fil-2
  • Species
    C. elegans (nematode)
  • Entrez Gene
    fil-2 (a.k.a. CELE_K12B6.3)
  • Promoter fil-2

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PstI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer M13 reverse
  • 3′ sequencing primer GFP R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJL1121 was a gift from Junho Lee (Addgene plasmid # 78188 ; http://n2t.net/addgene:78188 ; RRID:Addgene_78188)

  • For your References section:

    IRE-1 and HSP-4 contribute to energy homeostasis via fasting-induced lipases in C. elegans. Jo H, Shim J, Lee JH, Lee J, Kim JB. Cell Metab. 2009 May;9(5):440-8. doi: 10.1016/j.cmet.2009.04.004. 10.1016/j.cmet.2009.04.004 PubMed 19416714
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