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Addgene

pJL580
(Plasmid #78185)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 78185 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pPD95.75
  • Vector type
    Worm Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    rpy-1
  • Species
    C. elegans (nematode)
  • Entrez Gene
    rpy-1 (a.k.a. CELE_C18H9.7)
  • Promoter rpy-1

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)
  • 5′ sequencing primer M13 reverse
  • 3′ sequencing primer GFP R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJL580 was a gift from Junho Lee (Addgene plasmid # 78185 ; http://n2t.net/addgene:78185 ; RRID:Addgene_78185)

  • For your References section:

    Control of rapsyn stability by the CUL-3-containing E3 ligase complex. Nam S, Min K, Hwang H, Lee HO, Lee JH, Yoon J, Lee H, Park S, Lee J. J Biol Chem. 2009 Mar 20;284(12):8195-206. doi: 10.1074/jbc.M808230200. Epub 2009 Jan 20. 10.1074/jbc.M808230200 PubMed 19158078
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