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Addgene

MXS_BidirectionalCAG Notes

Addgene diagnostic digest

This gel depicts the NEB 1kb DNA ladder and a digestion of plasmid 78175 with SpeI and EcoRI-HF.

The expected pattern of bands was observed:

SpeI single digest: Bands at 5kb and 3 identical bands at 0.4kb

EcoRI-HF single digest: Bands at 4.2 and 2.0kb

SpeI/EcoRI-HF double digest: Bands at 2.0, 1.7, 1.4 and 0.4kb