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Addgene

pPICZalphaB-SapL3
(Plasmid #78171)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 78171 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pPICZα -B pichia vector
  • Backbone manufacturer
    ThermoFisher
  • Vector type
    Yeast Expression
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Bleocin (Zeocin), 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Saporin L3
  • Species
    Saponaria officinalis
  • Insert Size (bp)
    800
  • Tag / Fusion Protein
    • alpha factor (N terminal on backbone)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPICZalphaB-SapL3 was a gift from Vern Schramm (Addgene plasmid # 78171 ; http://n2t.net/addgene:78171 ; RRID:Addgene_78171)

  • For your References section:

    Soapwort Saporin L3 Expression in Yeast, Mutagenesis, and RNA Substrate Specificity. Yuan H, Du Q, Sturm MB, Schramm VL. Biochemistry. 2015 Jul 28;54(29):4565-74. doi: 10.1021/acs.biochem.5b00405. Epub 2015 Jul 14. 10.1021/acs.biochem.5b00405 PubMed 26091305
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